Protein Bodies at Early Stages of Development in High Lysine Mutant Notch-2 and Parent NP-113 Barley *

In barley parent NP-113, endospermic protein bodies originate on rough endoplasmic reticulum, either as electron Iransluscent vesicles or as very small, spherical, electron dense protein bodies. These are translocated to vacuoles for enlargement and subsequent storage. Endospermic protein bodies of Notch-2 high lysine mutant are either vacuoiar, or confined to distended cisternae of smooth endoplasmic reticulum. Vacuoiar protein bodies are of two types one, flocculent, which loosely fill up almost the entire vacuoiar space; two, spherical, relatively compact and granular. Protein bodies, confined to smooth endoplasmic reticulum are small, spherical, electron dense or electron transluscent. These protein bodies fuse to form electron dense proteinaceous masses which are deposited in the cytosol due to disruption of the confining smooth endoplasmic reticulum.