1Research Schools of Chemistry The Australian National University, Canberra, ACT 2601, Australia
2Biological Sciences, The Australian National University, Canberra, ACT 2601, Australia
* Corresponding author.
2 Present Address: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora-263 643, India
Cytokinin glucosides are routinely quantified as their aglycones produced by enzymic or chemical hydrolysis. It Is, however, important to be able to measure their levels per se. The present paper illustrates the use of desorption chemical ionisation mass spectrometry coupled with stable isotope dilution for the determination of intact, underlvatized N- and O-glucosyl conjugates of cytokinlns in Datura Innoxia crown gall tissue. A total of six glucosyl conjugates were determined; the two N-glucosides, zeatin-7-glucoside and zeatin-9-glucoside, were present In higher quantities than the O-glucosyl derivatives of zeatin, dihydrozeatin and their ribosides.
cytokinin glucosides, quantification, desorption chemical ionisation mass spectrometry, stable isotope dilution, crown gall tissue
