Plant Molecular Biology and Biochemistry Laboratory, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
*NBRI research publication number 394
We report here a simple procedure for the purification of the organelle DMA. Mitochondrial DNA from Sorghum and the chloroplast DNA from Populus and spinach were purified using this protocol. The method utilizes a quick centrifugation of the isolated organelle DNA through a two step CsCI density gradient for removal of small molecular weight nucleic acids which pose a major problem for getting clean restriction patterns. This method of purification can be adopted with any isolation procedure for organelle DNA.
organelle DNA, rate zonal centrifugation, purification, restriction
