Journal of Plant Biochemistry and Biotechnology
  • Year: 1993
  • Volume: 2
  • Issue: 2

Construction and Screening of Barley Genomic Library with a B1-Hordein Gene Probe*

  • Author:
  • Aruna Tyagi, I M Santha, S L Mehta1
  • Total Page Count: 2
  • Page Number: 125 to 126

Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-110 012, India.

1 Corresponding author.

* Part of Ph.D. Thesis submitted by AT to P.G. School, IARI.

Abstract

Barley genomic library from cv NP113 was made in a replacement vector EMBL-3. The titre was found to be 1.25 × 106 per μg of genomic DNA. The recombinants were screened using a B1-hordeln DNA probe. One clone contained a positively hybridizing 4 kb fragment.

Keywords

barley, genomic library, B1-hordein