The strain BYK1, capable of utilizing DAP or OX-DAPRO as a sole source of nitrogen and carbon was identified as Pseudomonas stutzeri by various microbial and biochemical tests. Transformation experiments showed that the OX-DAPRO utilization property is encoded by the plasmid. Restriction of the plasmid DNA followed by cloning of fragments and screening on OX-DAPRO medium led to isolation of a clone with DNA insert of ∼ 2.2 kb (designated as BSK8) which encoded OX-DAPRO degrading gene. The growth and OX-DAPRO utilization with this fragment was similar to wild type strain.
Lathyrus sativus, toxin degrading genes, soil-borne microbe, Pseudomonas, cloning
