Somatic Embryogenesis and Plant Regeneration from Tissue Cultures of Hyoscyamus muticus L.

Somatic embryogenesis and regeneration of plantlets was achieved in callus cuitures derived from cotyiedonary leaf pieces of Hyoscyamus muticus L on MS medium enriched with 2 mg/l 2,4-D and 0.5 mg/l BAP. For embryogenesis and organogenesis varying concentrations of NAA with or without BAP were added in the medium. Organogenesis was also achieved when callus was transferred to the hormone free medium.