Journal of Plant Biochemistry and Biotechnology
  • Year: 1994
  • Volume: 3
  • Issue: 1

Isolation and Pattern of Deposition of Proteins in Developing Mustard (Brassica juncea) Seeds

  • Author:
  • Kamal Dhawan, H S Nainawatee
  • Total Page Count: 3
  • Page Number: 55 to 57

Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hissar 125 004, Haryana, India

* Corresponding author

Abstract

Gel filtration of seed storage proteins of Indian mustard (Brassica juncea cv RH-30) by HPLC showed the presence of two major proteins of 112 and 26 kD. SDS-polyacrylamide gel electrophoresis showed that these proteins were composed of different subunits ranging from 12 to 66 kD. During seed development 26 kD proteins were the first to appear which was at 34 DAF stage while the 112 kD proteins appeared at 43 DAF stage. At different stages of seed development relative proportion of these proteins varied.

Keywords

Brassica juncea, high performance liquid chromatography, electrophoresis, seed storage proteins, seed development