Journal of Plant Biochemistry and Biotechnology
  • Year: 1994
  • Volume: 3
  • Issue: 1

Isolation and Characterization of Cell Lines Resistant to Crude Culture Filtrate of Fusarium in Chickpea

  • Author:
  • Surya Parkash1, Anuradha Sehgal2, Randhir Singh2, J B Chowdhury1
  • Total Page Count: 3
  • Page Number: 63 to 65

1Department of Genetics CCS Haryana Agricultural University, Hissar 125 004, India

2Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hissar 125 004, India

* Corresponding author

Abstract

Cell lines resistant to Fusarium oxysporum f. sp. Cicerl crude culture filtrate have been selected in Cicer arletlnum cv C235. Phenol content and the activities of tyrosine ammonia Iyase (TAL), phenylalanine ammonia Iyase (PAL) and D-1, 3-glucanase were increased by the ellcitor In callus cultures of Fusarium susceptible cultivar C235, resistant cultivar WR315 and a stable Fusarium resistant cell clone FC-3. The increase in phenols and glucanase activity, was more in selected cell line than in cv C235 and cv WR315, while the increase in activities of TAL and PAL showed reverse correlation.

Keywords

Cicer arietinum, Fusarium oxysporum, in vitro selection, phenols, enzymes