Journal of Plant Biochemistry and Biotechnology
  • Year: 1995
  • Volume: 4
  • Issue: 1

A Simple and Rapid Aqueous Method for the Purification of DMA from Wheat Chloroplast

  • Author:
  • Abhay K Singh, S Chauhan, G S Singhal
  • Total Page Count: 3
  • Page Number: 51 to 53

School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India

* Corresponding author.

Abstract

We report here a simple and rapid method for the purification of chloroplast DNA (ctDNA) from wheat (Triticum aestivum). It utilizes an aqueous procedure, which does not involve at any stage running of gradients. Due to use of DEPC which inactivates DNases activated by EDTA, the DNase action on crude chloroplast preparation containing ctDNA is avoided.

Keywords

aqueous method, ctDNA, purification, restriction, Southern, wheat