Journal of Plant Biochemistry and Biotechnology
  • Year: 1995
  • Volume: 4
  • Issue: 2

Sequence Homology of Two Indian Isolates of Cucumber Mosaic Virus at N-terminal Amino Acid Sequences of the Coat Protein Gene with CMV Strains

  • Author:
  • S K Raj, Q M R Haq, K M Srivastava, B P Singh
  • Total Page Count: 4
  • Page Number: 77 to 80

Abstract

The cDNAs representing coat protein gene (RNA4) of CMV-CR (Dianthus barbatus strain) and CMV-P (Physalis minima strain) were synthesized using random primers and cloned into Bluescript II KS (+) phagemid at Sma I site. The clones were partially sequenced and compared with published nucleotide sequences of selected CMV-strains. Comparison of 249 nucleotide sequences of 5′ coding region of CP gene and their putative products revealed the possibility of placing the strains into sub-group I of CMV.

Keywords

cucumber mosaic virus, coat protein gene, sequence homology