Journal of Plant Biochemistry and Biotechnology
  • Year: 1996
  • Volume: 5
  • Issue: 1

Plant Regeneration from Leaf Explants of Three Grain Legumes on Same Medium

  • Author:
  • Asha Kunjumon, V Ramesh Kannan, Yogesh T Jasrai
  • Total Page Count: 3
  • Page Number: 27 to 29

1Department of Botany, Faculty of Science, The M S University of Baroda, Vadodara 390002, India

* Corresponding author.

Abstract

The regeneration potential of leaves of moth bean, pigeonpea and grass pea was studied on Murashige and Skoog's (MS) medium supplemented with various combinations of auxins and cytokinins. Regeneration of shoots was obtained mainly on MS medium supplemented with high auxin and low cytokinin levels. However, frequency of response varied not only in the three legume species but also in their varieties studied. Roots were induced on regenerated shoots by transferring them to MS basal medium. Complete plant regeneration was observed in the three legumes in a short duration of 60–70 days.

Keywords

moth bean, pigeonpea, grass pea, regeneration, leaf explants