Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore 560 089, India
* Corresponding author.
Nitrogen-fixing bacterial isolate from the intercellular spaces of tomato root cortical cells was studied for the location of nif genes on the chromosomal or plasmid DNA. The bacterial isolate showed two plasmids of approximate molecular sizes of 220 and 120 kb. Klebsiella pneumoniae nifHDK probe hybridized with the chromosomal DNA and not with the plasmid DNA thereby showing that nif genes are localised on the chromosomal DNA.
chromosomal DNA, nif HDK probe, hybridization
