Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar 125 004, India
The production of Tsr factor by Rhizobium leguminosarum bv. viciae was influenced by low temperature (10°C) in the presence of seed exudate collected at 10°C and 25°C or naringenin (10μM). Root exudate collected at 25°C and naringenin induced Tsr factor in R. leguminosarum causing thick and short root phenotype and root hair curling and deformation of host root. Root exudate collected at 10°C also induced root hair curling but Tsr activity was low. Low temperature grown plants had poor nodulation, nitrogen fixation, nitrogen content and total biomass as compared to plants grown at 25°C. Rhizospheric application of naringenin partially alleviated the deleterious effect of low temperature on nodulation status and nodule efficiency.
naringenin, Pisum sativum, Rhizobium leguminosarumbv.viciae, symbiosis, temperature
