aDepartment of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri 413 722, India
bDivision of Biochemistry. Indian Agricultural Research Institute, New Delhi 110 012, India
*Corresponding author.
Nitrate reductase activity in the leaves of a number of plants after transfer from light to dark was assayed both by In vivo and in vitro methods. The initial activity persisted during the dark phase for a considerable length of time and declined gradually. After exposure to light again, the NR activity increased rapidly. The possibility of nitrate assimilation in complete darkness is discussed.
higher plants, light-dark mechanisms, nitrate reductase inhibition, Mg2+ ions
