Journal of Plant Biochemistry and Biotechnology
  • Year: 1999
  • Volume: 8
  • Issue: 2

Employing a Crude Toxin Preparation from Sarocladium oryzae as a Molecular Sieve to Select Sheath Rot-resistant Somaclones of Rice

  • Author:
  • R G Hernalatha1, S Jebaraj1, J A J Raja2, T Raguchander2, A Ramanathan2, R Sarniyappan2, P Balasubrarnanian3,
  • Total Page Count: 6
  • Page Number: 75 to 80

1Department of Agricultural Botany, Agricultural College and Research Institute, Madurai

2Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore

3Centre for Plant Molecular Biology, Tamil Nadu Agricultural University. Coirnbatore 641 003, India

*Corresponding author

Abstract

This study was undertaken to explore the possibilities of using in vitro techniques for selection of sheath rot-resistant somaclones of rice cv Co43. Oryza nivara and O. longistaminata possessing sheath rot-resistance were included in the study, with a view to comparing the reaction of the susceptible somaclones of O. sativa cv Co43 to a crude toxin preparation from culture filtrate of the pathogenic fungus, Sarocladium oryzae. A few somaclones of Co43, selected on the crude toxin preparation, regenerated into plantlets and their reaction to the toxin was reconfirmed up to R1 generation.

Keywords

Sarocladium oryzae, rice sheath rot, toxin, somaclonal variation, disease resistance