Journal of Plant Biochemistry and Biotechnology
  • Year: 1999
  • Volume: 8
  • Issue: 2

Multiplication of Eucalyptus citriodora (L) Hook Through Shoot Bud Induction on the lnternodal Portions of Mature Tree Explants

  • Author:
  • Yogesh T Jasrai, A Remakanthan, C H Pandya, J Subramani, D P Bhatt
  • Total Page Count: 2
  • Page Number: 103 to 104

*Corresponding author.

Abstract

Nodal explants of Eucalyptus citriodora responded better for high frequency bud break and fast growth in liquid agitated cultures with 4.4 pM benzyladenine (BA) and 5.37 μM a-naphthalene acetic acid (NAA) rather than on semisolid medium. On transfer to MS medium with 1.11 pM BA and 5.37 μM NAA the sprouted axillary buds showed further elongation growth alongwith a slight callus and formation of globular structures on the internodal regions. The anatomy of these globular structures revealed that they are shoot buds. These buds elongated into shoots on MS medium with low levels of BA.

Keywords

Eucalyptus citriodora, micropropagation, de novobuds, low temperature treatment