Journal of Plant Biochemistry and Biotechnology
  • Year: 1999
  • Volume: 8
  • Issue: 2

Withania somnifera (Ashwagandha): Regeneration through Meristem Culture

  • Author:
  • Nilesh P Teli1, Nilima M Patil, Hema M Pathak, Sanjeevani R Bhalsing, Vijay L Maheshwari
  • Total Page Count: 3
  • Page Number: 109 to 111

Department of Biochemistry, School of Life Sciences, North Maharashtra University, Jalgaon 425 001, India

Abstract

High frequency of callus induction was achieved from shoot tip explants (80%) of Withania somnifera (Hindi name-Ashwagandha) as compared to leaf explants (70%), on MS medium supplemented with IAA (56μM) and Kinetin (56μM). When hormone-free MS medium was fortified with vitamins [thiamine. HCI (3μM), nicotinic acid (40.7μM) and pyridoxine. HCI (24.3μM)], it was found suitable for complete plant regeneration through meristem culture.

Keywords

ashwagandha, callus culture, medicinal plant