M S Swarninathan Research Foundation, lllrd Cross Street, Tararnani, Chennai 600 113, India
*Corresponding author
AbbreviationsBAP
benzylarninopurine
2,4-D2,4-Dichlorophenoxyacetic acid
IBAindole-3-butyric acid
Knkinetin
MSMurashige & Skoog
NAAα-naphtttaleneacetic acid
SEstandard error
Curculigo orchioides Gaertn is an important medicinal plant of commercial value. A protocol for indirect regeneration from the leaf explants was developed. The leaf explants on MS (Murashige & Skoog) basal medium fortified with 6-benzylaminopurine (9μM) gave rise to proliferating green callus. The calli regenerated shoots on subculturing in the MS medium with 1.1 μM 6-benrylaminopurine which was the optimum concentration for multiplication. Rooting was observed in MS medium supplemented with a-napthaleneacetic acid (5.3μM), indole-3-butyric acid (1.2μM), and on MS basal medium free of plant growth regulators. The rooted plants were hardened and transferred to field with 80% survival.
Curculigo orchioides, indirect organogenesis, in vitropropagation, medicinal plant
