Cloning of a Gene Sequence for ω-3 Desaturase from Brassica juncea

cDNA was synthesized from RNA isolated from developing seeds of Brassica juncea cv Pusa Jai Kisan at 25–45 days after flowering and partial cDNA sequences encoding ω-3 desaturase gene were PCR amplified using specifically designed primers. The amplified products were cloned, and three of the clones subjected to partial sequence analysis. The clones showed high homology to ω-3 desaturase sequence from other sources. A genomic clone corresponding to ω-3 desaturase was isolated from the genomic library of B. juncea constructed in λEMBL-3 vector using one of the PCR clones.