1Department of Botany, University of Delhi, Delhi 110 007, India
2Miranda House, University of Delhi, Delhi 110 007, India
3SSN College, Alipur, Delhi, India
*Corresponding author.
+Present Address; Department of Botany, KLDAV College, Roorkee, District Haridwar (UP)
Female gametophyte of Cycas circinalis and Zamia integrifolia and embryo of C. circinalis were cultured in vitro on MS medium supplemented with various auxins and cytokinins. In female gametophyte cultures of C. circinalis and Z. integrifolia, auxins (NAA, 2,4-D) alone resulted in callus formation. However, when auxin was used in Combination with BAP or Kn, nodulation was observed. Embryos of C. circinalis grown on a medium containing 2,4-D and BAP showed organogenic response and bulbil-like structures.
Cycas, Zamia, organogenesis, embryo, female gametophyte, tissue culture
