1Tata Energy Research Institute, Darbari Seth Block, Habitat Place, Lodhi Road, New Delhi, 110 003, India
2Centre for Biotechnology, Faculty of Science, Hamdard University, Hamdard Nagar, New Delhi 110 062, India
*Corresponding author. Email: mailbox@teri.res.in
The in vitro bulblet multiplication of Lilium sp. (Asiatic hybrids) enhanced significantly by the application of polyamines than when cultured on medium containing high sucrose and auxin (NAA). Of the two polyamines tested, spermine was most effective. Maximum multiplication fold was 2.89 for cultivar ‘Gran Paradiso’ at 150 μM spermine and 2.78 fold for cultivar ‘Sanciro’ at 50 μM. Putrescine also stimulated bulblet formation and multiplication to the tune of 2.56 and 2.61 fold at 50 μM as compared to control where it was 2.13 and 2.42 fold for cultivars ‘Gran Paradiso’ and ‘Sanciro’, respectively. Though polyamines were effective in enhancing multiplication rates on an average of 26% in a year, growth of bulblets remained unaffected.
Asiatic hybrids, bulblet multiplication, polyamines, spermine
