1Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444104 (M.S.), India
*Email: aggathe108@gmail.com
Trichodermaviride mutants which were applied against soil borne plant pathogens were found effective against these pathogens after application of different concentrations chemical mutagens of Ethyl Methyl Sulphonate (EMS) and Hydroxyl Amine (HA) i.e. @ 50, 100,150,200 and 250 µl/ml at time interval of 60 minutes. Ten T.viride mutants were obtained and tested for their antagonistic activities invitro. Efficient mutants were tested up to six generation to check their stability. Among the mutants, four stable mutants viz.,TvME-4 (T4), TvMH-8 (T8), TvME-3 (T3) and TvMH-9 (T9) were proved as effective antagonists against Sclerotiumrolfsii,where as In case of Rhizoctoniabataticola the mutants TvME-4 (T4), TvMH-8 (T8), TvME-3 (T3) and TvMH-10 (T10) showed maximum growth inhibition and Fusariumudum was also inhibited by mutants TvME-3 (T3), TvME-4 (T4), TvMH-9 (T9) and TvMH-8 (T8).
Mutation, Trichoderma viride, Ethyl Methyl Sulphonate (EMS), Hydroxyl Amine (HA), Sclerotium rolfsii, Rhizoctonia bataticola, Fusarium udum
