1Plant Pathology Unit, Tamil Nadu Rice Research Institute, Aduthurai, Thanjavur District - 612 201
2Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-3
3Department of Plant Pathology, Uttar Banga Krishi Vishwavidyalaya, Pundibari, Cooch Behar District - 736 165
4Department of Plant Breeding, Agricultural College and Research Institute, TNA University, Madhurai - 625 104
5Department of Plant Breeding, Sugarcane Research Centre, TNAU, Cuddalore - 607 001 (T.N.)
*Corrresponding author e-mail: ahila.devi1@gmail.com
Online Published on 06 June, 2025.
Black gram is an important pulse crop in India as well as in South East Asia. The main reason for the fluctuation in the average yield is various biotic stresses which results in 5–100% yield loss. Among these, Yellow mosaic disease (YMD) caused by Mungbean Yellow Mosaic Virus (MYMV), is a most destructive disease of blackgram in the entire South East Asia. This disease is transmitted by white fly (Bemisia tabaci Gennadius) and not by mechanical inoculation or by seed. The main objective was carried out identify the vectors involved in the transmission. The whitefly was transmitted by persistent manner. Virus vector relationship revealed that the minimum of 5 adults of whitefly with an acquisition access period of 1 month and inoculation access of 5 min could able to transmit the yellow mosaic disease. Study indicated that the degenerate primers used in the present investigation amplified approx. 900 bp amplicon of virus. The per cent detection of virus from the seedcoat, cotyledon, embryo and whole seed was 100 per cent in samples tested showed presence of virus.
Black gram, Seed Borne, Transmission, Yellow mosaic
