Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, 600 025, Tamil Nadu, India.
The capability of the plant pathogenic fungus Fusarium oxysporum f. sp. vasinfectum to elaborate extracellular lipases in the submerged cultures was assyed on Tween-20 agar. The pathogen produced 95 U/mL of lipase after 7 d incubation at 25 DC under submerged fermentation. Glucose was found to be the best carbon source whereas sodium nitrate proved to be the best nitrogen source for lipase activity. The optimum lipase activity was achieved at. pH 7 and 25 DC. Tween-80 and olive oil were found to be the best inducers of the enzyme.
Fusarium oxysporum f. sp. vasinfectum, phytopathbgen, submerged fermentation, inducers
