Isolation, Identification and Documentation of Efficient Chitinase Enzyme Production Ability Strains of Trichoderma

Trichoderma isolates varied among themselves with respect to production of protein, isolate Tv-23 produced highest of 129.58 mg/g protein followed Th-13 and Tv-21 with 127.14 and 125.5mg/g protein in culture fllterates.In crude protein estimation all the Five isolates produced band with 36 and 45 kDa, however they are more pronounced in Th-13, Tv-23 and Tv-21. With respect to total Chitinase concentration in crude sample isolates Th-10 and Tv-23 produced more thick and stretched band compared to Th-13 and Tv-21. T. harzianum isolates produced more chitinase in spite of their poor total production. When culture filtrates were dialyzed and concentrated highest 7563 units of enzymes were produced by Tv-10 with 13.08 units/mg of protein indicating its superiority in producing the enzymes. The enzyme purified was found to be endochitinase as it also reduced the turbidity of the chitinase with 41 molecular weight. Varying degree of substrate utilization by the biotypes of Trichoderma indicates their different mycoparasitic potentiality.