Journal of Plant Disease Sciences

Papaya Ringspot Virus P (PRSV-P) is a widely prevalent plant virus infecting Carica papaya causing substantial crop loss and at times devastating enough to wipe out entire plantations. Unlike other plant pathogens, there are no direct methods available to control the pathogen. Methods for detection and identification of viruses both in plants and vectors play a critical role in virus disease management. The present study was therefore undertaken to standardize a DOT-Enzyme Linked Immunosorbent Assay (DIBA) and plate-Enzyme Linked Immunosorbent Assay (ELISA) for the detection of PRSV. The ultrapurified virus was confirmed electron microscopically as flexuous rod shaped particles measuring 700 X 12–13 nm. A titer of 1:50 of antigen, 1:16000 of primary antibody and 1:2000 of secondary antibody was standardized for direct and indirect DIBA. Atitre of 1:50 dilution of antigen, 1:8000 of primary antibody and 1:2000 of secondary antibody was standardized for direct plate ELISA and 1:10 dilution of antigen, 1:16000 of primary antibody and 1:2000 of secondary antibody was standardized for indirect plate ELISA with an optimum optical density of 1.134 and 1.016 at 405 nm respectively.