Agricultural Research Journal
Open Access
SCOPUS
  • Year: 2015
  • Volume: 52
  • Issue: 2

Nutritional and physiological requirements for growth and sporulation of Alternaria radicina causing blight of carrot

Department of Plant Pathology, Punjab Agricultural University, Ludhiana-141004

*Corresponding author: asjagdey@pau.edu

Online published on 18 May, 2015.

Abstract

Alternaria radicina (Kuhn) Groves and Skolko, the causal agent of blight of carrot, was isolated from infected leaves of carrot seed crop. Different semi-synthetic and synthetic media namely, potato dextrose agar, carrot leaf agar, umbel extract agar, oatmeal agar, corn meal agar, Richard's agar and Czapek's dox agar were evaluated for growth and sporulation of the pathogen. Carrot leaf agar supported maximum vegetative growth and sporulation followed by umbel extract agar but least growth and sporulation was recorded in Czapek's Dox agar medium. The optimum temperature for maximum vegetative growth and sporulation was observed at 25°C. Both mycelial growth and sporulation decreased with increase in temperature to 30°C. The colony growth and sporulation ceased altogether at 35°C. At lower range of temperature, the fungal growth and sporulation were decreased. Optimum pH for maximum vegetative growth of A. radicina was 6.0, whereas, for getting maximum sporulation pH 7.0 was found to be optimum.

Keywords

Alternaria radicina, Blight, Carrot, Colony growth, Culture media, pH, Sporulation, Temperature