Studies on Nutritional Requirements of Different Strains of King Oyster Mushroom (Pleurotus eryngii)
Abstract
Pleurotus eryngii strains (DMR-P-120, DMR-P-135 and DMR-P-257) were evaluated for growth on solid and liquid media. Malt agar medium (natural), potato dextrose agar (semi-synthetic) and Richard's medium (synthetic medium) were the best media among different agar media evaluated. In liquid culture, semi-synthetic Sabouraud's medium performed the best with a yield of 17.40 to 20.80 g/L of culture media. Among the various carbon and nitrogen sources tested in synthetic medium (Richard's medium), mannitol proved to be the best carbon source yielding 7.0 to 8.07 g/L for the three strains while, L-aspargine was the preferred nitrogen source for strains DMR-P-135(9.07 g/L) and DMR-P-257(9.73 g/L) though sodium nitrate was exceptionally utilized by strain DMR-P-120 (8.07 g/L). Among different macro-elements tested, potassium supported maximum growth. Whereas among the five micro-elements, molybdenum supported maximum growth of strains DMR-P-120 and DMR-P-135, while manganese was found to be best for the strain DMR-P-257.
Keywords
Biomass, Growth, Nutritional requirements, Pleurotus eryngii strains