In vitro propagation of strawberry (fragaria x ananassa duch.) cv. chandler

A micropropagation method is described for strawberry (Fragaria x ananassa Duch.) Cv. Chandler. Vegetative buds were surface sterilized with HgCl₂ (0.1%) for 3 minutes which gave minimum contamination with maximum culture establishment. Of various treatment combinations, Murashige and Skoog (MS) medium + BAP 1.00 mg/l with GAs 2.00 mg/l resulted in maximum establishment of cultures in lesser time. MS medium + BAP 2.00 mg/l + GAj 2.00 mg/l gave maximum multiple shoots. Maximum rooting was obtained on MS medium (half strength) supplemented with lBA 1.00 mg/l and activated charcoal 200 mg/l.