1Department of Parasitology, Madras Veterinary College, Chennai-600 007, India
2Central University Laboratory, Directorate of Centre for Animal Health Studies, TANUVAS, Madhavaram Milk Colony, Chennai-600 051.
*Present address. No. 533/C4, Vasantha Nagar East St., Vellipatinam, Ramanathapuram-623 504, India
*Part of M.V.Sc. Thesis by the first author, Tamil Nadu Veterinary and Animal Sciences, Chennai-600 051
An attempt was made to produce excretory and secretory (E/S) antigen of Toxocara canis larvae. The larvae of T. canis were experimentally hatched using 37% sodium hypochlorite and were maintained in RPMI-1640 medium with antibiotics in an incubator at 37°C, 95% humidity and gassed with 5% CO2. The culture fluid containing E/S antigen was dialysed, ultrafiltered and lyophilized.
Toxocara canis, In vitro cultivation, Metabolic antigen
