Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
*Part of M.V.Sc. thesis TANUVAS E-mail: Vetarul@rediffmail.com
The crude midgut antigen of Rhipicephalus haemaphysaloides characterised by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed 11 protein bands with Coomassie blue staining ranging from 20.1 to 80.4 kDa. Partially purified midgut antigen resolved by SDS-PAGE revealed 3 protein bands at 35.9, 43.0 and 53.2 kDa while with silver staining, 8 protein bands were seen ranging from 20.1 to 58.3 kDa. Western blot studies of crude midgut antigen of R. haemaphysaloides revealed 5 immunogenic polypeptides at 25.3, 29.0, 35.4, 43.0 and 66.0 kDa, while the partially purified midgut antigen revealed 4 immunogenic proteins at 25.4, 35.9, 43.0 and 58.4 kDa.
Midgut, Antigen, SDS-PAGE, Rhipicephalus haemaphysaloides, Western blot
