In vitro effect of Allium sativum on lactate dehydrogenase (LDH) of Haemonchus contortus was studied. A. sativum significantly inhibited the LDH activity catalysing both oxidation of lactate and reduction of pyruvate. At 1% concentration after 8h exposure, the inhibition of LDH catalysing the oxidation of lactate was 74.57% and the inhibition of LDH catalysing the reduction of pyruvate was 38.41%. This results in the debilitation of pyruvate and the accumulation of lactate. Consequently, the generation of ATP is affected and the energy-deprived worms unable to sustain themselves in situ get eliminated from the host.
Haemonchus contortus, Lactate dehydrogenase, Allium sativum
