Morphological and molecular identification of Teladorsagia (Ostertagia) circumcincta from goats

Helminths collected from abomasum of goats were identified morphologically as well as by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as Teladorsagia circumcincta. Male copulatory bursa was having bursal membrane and a small dorsal lobe. The ends of the spicules were bifurcated and ending with large knob like structure and small acute process. The female has 4–5 annular striations on tip of the tail. PCR was performed with adult genomic DNA for amplification of 770 bp fragment of β-tubulin gene implicated in benzimidazole resistance. The result of restriction enzyme analysis of this fragment with Rsa I enzyme was clearly indicative of T. circumcincta.