Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Vishwavidyalaya, Anjora, Durg-491 001, India.
Influence of media type, temperature and pH of media were investigated with Chhattisgarh isolate of nematode trapping fungi, Arthrobotrys oligospora and Duddingtonia jlagrans. In corn meal agar, full radial growth was achieved for A. oligospora on day 6 after plating and for D. jlagrans it was on day 7. Next medium of choice was nutrient agar where both the fungi obtained considerable growth. Poor growth and scattered radial growth for both A. oligospora and D. jlagrans were observed in Sabouraud glucose agar, potato dextrose agar and water agar, respectively. Though significant growth of both the fungi was recorded between 18–34°C, the optimum temperature observed for their growth was 26°C. Optimal pH required for growth of both the species was 6 and further acidic pH (4 and 5) adversely affected their in vitro growth.
Arthrobotrys oligospora, Duddingtonia flagrans, In vitro growth, Media, pH, Temperature
