Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
1Present address: Faculty of Veterinary Sciences and Animal Husbandary, SKUAST-K, Shuhama Alusteng Srinagar-190 006, Post Box No. 135 GPO, Srinagar.
An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against Trypanosoma evansi in dromedary camel was standardized using three types of purified antigens viz., GFAE1 (gel filtration anion - exchange fraction I), GFAE2 (gel filtration anion-exchange fraction II) and GPA (affinity purified glycoprotein antigen). A total of 90 field serum samples of camels were screened with ELISA using GFAE1, GFAE2 and GPA as test antigens and out of them 74(82.22%), 69(76.66%) and 65(72.22%) were found positive for T. evansi antibodies, respectively. The purified antigen GFAE1, (high molecular weight proteins) was found superior to other purified antigens with the highest serodetection (82.22%), followed by GFAE2 (low molecular weight proteins) (76.66%) and GPA (glycoproteins) (72.22%).
Camel, Ab-ELISA, Trypanosoma evansi, Detained antigens
