Centre of Advanced Studies, Department of Veterinary Parasitology, Veterinary College, KVAFSU, Hebbal, Bangalore-560 024, India.
*Corresponding author
Part of M.V.Sc thesis of the first author Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar.
Detection of echinococcosis in dogs by copromicroscopy is not confirmatory since Taenia and Echinococcus eggs are morphologically indistinguishable. Therefore, coproantigen detection by counter immuno-electrophoresis (CIEP) was evaluated for this purpose. Adult Echinococcus granulosus worms were obtained from intestine of necropsied positive dogs and excretory-secretory antigen was obtained by culturing the adult worm in medium 199 (pH 7.4). The hyperimmune serum was raised against this antigen in rabbits. Supernatant of the faeces of dogs was used for coproantigen detection. CIEP was carried out using tris-borate buffer (pH 8.0) at a constant current of 8mA per slide for 60 minutes. Out of 62 samples screened, 3 were found to be positive for Echinococcus granulosus coproantigens by CIEP.
Dog, Echinococcus granulosus, Coproantigen, CIEP
