Journal of Veterinary Parasitology

SCOPUS
  • Year: 2011
  • Volume: 25
  • Issue: 2

Molecular cloning and comparative sequence analysis of open reading frame of SAG2 gene of Toxoplasma gondii

  • Author:
  • Harkirat Singh, A.K. Tewari1, A.K. Mishra1, B.R. Maharana2, J.R. Rao3, O.K. Raina1
  • Total Page Count: 6
  • DOI:
  • Page Number: 107 to 112

Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, India.

1Division of Parasitology, IVRI, Izatnagar.

2Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat-362 001.

3National Academy of Agricultural Research management, Rajendra Nagar, Hyderabad.

Abstract

In view of the potential prospect of recombinant proteins for application in specific and sensitive immunodiagnosis as well as prophylaxis, emphasis is laid on the heterologous expression of several target recombinant proteins of Toxoplasma gondii and their characterization for specific applications. The T. gondii surface antigens are important for application in sensitive immunodiagnostic tools as well as for induction of protective immune response. The present communication describes the amplification of 561 bp SAG2 open reading frame (ORF) from two local isolates of T. gondii (Izatnagar and Chennai) as well as from RH strain and their subsequent cloning and sequence analysis. The comparative sequence analysis of the SAG2 ORF revealed 100% sequence homology between the RH strain, published SAG2 sequence of RH strain and Izatnagar isolate of the parasite, whereas substitution of single nucleotide noted at position 462 in Chennai isolate. Three-dimensional model of the computer simulated recombinant mature (truncated) SAG2 revealed that the porotein is monomer in nature with a single domain and the protein conformation consisted of beta sheets with intermittent alpha helices.

Keywords

Cloning, Homology, SAG2, Toxoplasma gondii