Several PCR assays using different primer sets have been suggested for the diagnosis of toxoplasmosis in animals and man. High repeatability of selected gene is desired for better visualization of amplified product. A 200–300 fold repetitive 529 bp PCR assay was tested for its efficacy in amplifying the DNA of theoretically lowest number of tachyzoites of
Analytical sensitivity, Toxoplasma gondii, 529 bp gene, PCR
