Application of forensic entomology for judging the different poisoning cases in animals: In vitro studies in wistar rats (Rattus norvegicus)

In-vitro studies in rats were undertaken on application of forensic entomology for judging the different poisoning cases in animals. The poisons studied were 1) Arsenic (Sodium arsinate) -metal; 2) Dimethorate (Rogar) –Organophosphate; 3) Benfuracarb – Carbamet; 4) Deltamithrin -synthetic pyrethroid along with control group. Flies belonging to Musca sp found arrived quite earlier (0.18 hr) on arsenic poisoning, delayed on deltamithrin (0.59 hr) and benfuracarb (2.5 hr) poisoned rat carcasses as compared to control (0.28 hr), while, on dimethorate poisoned carcasses of rats not a single species of fly turned at any time. Analogous type of observations were recorded for Chrysomyia¹, Chrysomyia² and Sarcophaga flies in different poisoning cases and their arrival were either earlier or delayed. In general it was concluded that in suspected cases of poisoning, absence of flies on carcass indicated organophosphate poisoning. In delayed appearance of flies, metal (Arsenic), carbamet (Benfuracarb) or synthetic pyrethroid (Deltamithrin) poisoning can be suspected. Non arrival of Chrysomyia² flies was suggestive of benfuracarb poisoning. In many poisoning cases it was observed that, Sarcophaga flies arrived on carcass as per the schedule, whereas other species of flies do not followed the schedule.