Pathobiochemical changes in Trichogaster fasciatus fish infected with progenetic metacercariae of Clinostomum complanatum

Clinostomum complanatum infection in Trichogaster fasciatus fish is very common in Northern India and the prevalence in Aligarh has been found to be 100% in April, June, September and October months. Pathobiochemical changes in fishes were assayed using GOT, GPT, acid and alkaline phosphatase (AcPase and ALPase) enzymes according to the standard protocols. The results showed elevated levels of all the pathological marker enzymes in the liver of infected fish as compared to the liver of normal fish. EDTA and iodoacetamide showed differential effects on the pathological marker enzymes. GPT and ALPase activities of infected liver were inhibited by 93% and 75% respectively with iodoacetamide while EDTA showed mild inhibitory effect on GOT and GPT activities. Polypeptide profile of infected and normal liver tissues revealed a total of 28 and 30 polypeptides respectively, with an apparent molecular weight in the range of 16–180 kDa. Further, on comparing the polypeptide profile it was observed that 100 kDa, 96 kDa and 48 kDa bands which were present in the normal liver were absent from infected T. fasciatus liver. It is concluded that enzyme levels and polypeptide profile of infected liver tissue is altered as a result of C. complanatum progenetic metacercaria infection in T. fasciatus.