1Division of T.A.H, IVRI, Mukteshwar, Nainital, Uttarakhand
Network Programme on Gastro Intestinal Parasitism, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India
*Corresponding author. Email: drarvindivri@rediffmail.com
Online published on 18 December, 2018.
The present study describes characterization of hmcp-5 gene, an isotype of cysteine proteinase of Haemonchus contortus. The immunodominant region of hmcp-5 gene (912 bp) of Indian strain of H. contortus. Coding 303 amino acids was PCR amplified from cDNA, cloned and characterized. Sequence analysis revealed theoretical molecular mass of 33.5 kDa with 303 deduced amino acids. The gene was found to have 98.2% identity with mRNA sequence for hmcp-5 cysteine proteinase gene of UK strain of H. contortus. However, the deduced amino acid sequence had only 96.1% homology with UK strain. There were 11 amino acid changes observed between these two strains. Further expression and functional studies of HMCP-5 protein of H. contortus are warranted to assess the immunoprophylactic potential.
Haemonchus contortus, Cysteine proteinase, hmcp-5, Molecular characterization
