Susceptibility status of Hyalomma anatolicum ticks collected from Western Punjab against cypermethrin

Susceptibility status of Hyalomma anatolicum ticks collected during April 2014 to October 2014 from eight districts of Western Punjab was tested against cypermethrin. The slope of mortality, lethal concentration for 50% (LC₅₀), 95% mortality (LC₉₅) values and resistance ratio (RR) were determined from the regression graphs of probit mortality of ticks plotted against log values of increasing concentrations of cypermethrin using the larval packet test (LPT). Values of the coefficient of determination (R²) for LPT ranged from 0.791 to 0.953 indicating the statistical model a good fit. Among the various field isolates, LC₅₀ and LC₉₅ values against cypermethrin ranged from 4.18–57.77 and 41.37–354.81 ppm, respectively. The value of RR₉₅ ranged from 0.12–1.01 indicating a susceptible status in all field isolates.