Department of Veterinary Pathology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600007, Tamil Nadu, India
*Correspoinding author. Email: nagavet@gmail.com
**Department of Veterinary Parasitology
*Part of M.V. Sc. thesis submitted by the first author to the Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu
A total of 103 intestines of sheep from slaughter house at Perambur, Chennai, Tamil Nadu were examined for the pathological lesions due to parasitic infections. Hook worm infection due to Bunostomum trigonocephalum could be identified in three animals. Grossly, infected intestine showed multiple petechiae in the ileal mucosa which were visible from serosal surface. Adult parasites were seen attached to the mucosal surface around which petechiae and oedema were noticed. Histopathologically, the haematoxylin and eosin (H&E) stained sections of jejunum revealed desquamation, congestion and haemorrhage with severe infiltration of eosinophils, plasma cells and lymphocytes. For molecular identification of Bunostomum species, polymerase chain reaction (PCR) was carried out targeting ITS2 region. DNA isolated from both the faecal eggs and adult worms showed specific 171 bp sized amplicon.
Sheep, Hook worms, Bunostomum trigonocephalum, Pathological lesions, Polymerase chain reaction
