Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, India
*Corresponding author. Email: viks.sudan@gmail.com
Online published on 10 February, 2021.
The study was designed with the objective to find out phylogenetic relationship between Anaplasma marginale isolates from Mathura, Uttar Pradesh with those from rest of India and abroad. For this purpose, a total of five isolates were characterized for major surface protein (msp5) gene. The sequences obtained were submitted into NCBI and corresponding accession numbers (MT449417-MT449421) were obtained. Subsequently, these sequences were compared with other Indian and global sequences of Anaplasma spp. The sequences of A. marginale formed a major clade on phylogenetic tree, wherein, the present sequences shared complete nucleotide homology with sequences from Kerala, India and with those originating from China, Germany, Egypt, Japan, Brazil, Sri Lanka, Thailand and Czech Republic. However, they differed considerably from a selected number of sequences that formed a sub group on the major A. marginale clade. This subgroup consisted of a sequence from Gujarat, India, besides some sequences from China, Egypt and Kenya. Sequences of A. centrale, A. ovis and A. phagocytophilum formed separate clades on the phylogenetic tree. Sub-grouping of Indian sequences of A. marginale showed existence of genetic diversity within the Indian isolates.
Anaplasma marginale, Cattle, Msp5 gene, Phylogenetic characterization, Sequencing
