All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin - Branch of the Federal State Budget Scientific Institution «Federal Scientific Center - All-Russian Scientific Research Institute of Experimental Veterinary Medicine K.I. Skryabin and Y.R. Kovalenko, The Russian Academy of Sciences, B. Cheremushkinskaya Street 28, 117218, Moscow, Russia
*Corresponding author. Email: Evgenia.sidor@gmail.com
The glycogen content in parasites, determined by spectrophotometric method, can be used as an additional criterion for assessing the effectiveness of anthelmintic drugs. In this study, the effect of albendazole and triclabendazole, drugs from the benzimidazole group, on the glycogen levels of adult Fasciola hepatica was investigated in Wistar rat model. Re-administration of anthelmintics resulted in a more significant effect on the carbohydrate and energy metabolism of Fasciola by 48 h, as evident by the lowest glycogen content during the experiment: 0.019 ± 0.003 mg/mg (triclabendazole) and 0.021 ± 0.004 mg/mg (albendazole) as compared to the control 0.045 ± 0.001 mg/mg at 22 ± 1°C. A decrease in the glycogen levels in flukes after therapy was significant (P < 0.05), while no significant differences were found between the two groups receiving different anthelmintics (P > 0.05). This study shows the degree to which flukes are susceptible to anthelmintics and can be useful in the development and evaluation of treatment regimens against F. hepatica and related parasites.
Glycogen, Triclabendazole, Albendazole, Fasciola hepatica, Spectrophotometric method
