1Department of Veterinary Parasitology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal, Bengaluru – 560024, India
2Department of Biochemistry, Veterinary College, Bengaluru, KVAFSU
3Institute of Animal Health and Veterinary Biologicals, Bengaluru
4Department of Veterinary Microbiology, Veterinary College, Bengaluru, KVAFSU
5Department of Animal genetics and breeding, Veterinary College, Bengaluru, KVAFSU
*Corresponding author E-mail: sindhooradivakar45@gmail.com
Online Published on 14 January, 2026.
A study was undertaken to develop a paper-based colorimetric loop-mediated isothermal amplification (LAMP) assay for the diagnosis of bovine tropical theileriosis. The hydroxy naphthol blue (HNB) wet LAMP assay was standardized for diagnosis of Theileria annulata in cattle. Further, the optimized wet LAMP assay was performed on a suitable paper substrate for the standardization of HNB paper LAMP with required modifications. The drying of colorimetric LAMP reagents on paper substrate allowed the transport of the ready-to-use LAMP kits to areas where the cold chain is not available and it can be employed for point-of-care diagnostics because of its single step operation.
Theileria annulata, Paper-LAMP, Hydroxy Naphthol Blue, Bovines
