Investigation of the influence of alpha-tocopherol on copper, iron, zinc and manganese levels in liver following chronic exposure of flubendiamide and copper to wistar rats

Present study was designed to examine the effects of alpha-tocopherol on the levels of Copper (Cu), Iron (Fe), Zinc (Zn) and manganese (Mn) in liver of copper and/or flubendiamide exposed (90 days oral) Wistar rats. Fifty four male Wistar albino rats were randomly divided into nine groups containing six animals in each: groups I (deionized water), II (corn oil) and III (α-tocopherol @ 100 mg/kg) served as negative controls while the remaining six groups, namely-IV (copper sulphate-@ 33 mg/kg), V (flubendiamide-@ 200 mg/kg), VI (flubendiamide-@ 200 mg/kg + copper sulphate- @ 33 mg/kg), VII (copper sulphate-@ 33 mg/kg + α-tocopherol-@ 100 mg/kg), VIII (flubendiamide-@ 200 mg/kg + α- tocopherol-@ 100 mg/kg) and IX (flubendiamide-@ 200 mg/kg + copper sulphate-@ 33 mg/kg + α-tocopherol-@ 100 mg/kg) were treatment groups. Animals were humanly sacrified on 91^st day. Cu, Fe Zn, and Mn were estimated in liver by Atomic Absorption Spectrophotometer. Copper and iron levels were markedly higher in copper, flubendiamide alone and combined exposed groups while manganese level was low in the xenobiotics exposed groups. No significant alteration was observed in level of zinc in any of the xenobiotics exposed as well as xenobiotics + α-tocopherol treatment groups. α-tocopherol-treatment improve the values of copper, iron, zinc and manganese towards the levels of control groups indicates antioxidant potential of α-tocopherol.