1Department of Veterinary Pathology, College of Veterinary and Animal Sciences, GB Pant University of Agriculture and Technology, Pantnagar-263 145
Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences, GB Pant University of Agriculture and Technology, Pantnagar-263 145
*Corresponding author e-mail: munishbatra74@rediffmail.com
Online published on 8 April, 2021.
The present study was planned to evaluate the pathological changes following oral administration of methanolic extract of Ajuga macrosperma in rats treated with cyclophophamide (CP). Forty eight (48) Wistar rats of either sex were divided randomly into eight groups of 6 rats each viz. groups I, II, III, IV, V, VI, VII and VIII. Group I served as control. Group II rats were administered CP @ 100 mg/kg b wt, orally on 9th and 16th day, group III was given levamisole @ 50 mg/kg b wt orally for 28 days, group IV was administered CP @ 100 mg/kg b wt orally on 9th and 16th day and levamisole @ 50 mg/kg b wt orally daily for 28 days, Group V was given methanolic extract of Ajuga macrosperma (MEAM) i.e. MEAM-1 @ 100 mg/kg b wt orally for 28 days, group VI was given MEAM-2 @ 200 mg/kg bwt, orally for 28 days, group VII was administered MEAM-1 @ 100 mg/kg b wt orally for 28 days and CP @ 100 mg/kg b wt orally on 9th and 16th day, group VIII was given MEAM-2 @ 200 mg/kg bwt, orally for 28 days and CP @ 100 mg/kg b wt. Histopathological studies revealed severe congestion of large and small blood vessels, severe sinusoidal congestion leading to loss of sinusoidal spaces, Kupffer cell proliferation, accumulation of mononuclear cells around many congested blood vessels, severe degeneration and swelling of hepatocytes throughout the parenchyma in liver; severe depletion of lymphoid cells, reticular cell hyperplasia, and depletion of red pulp and presence of hemosidrin pigment in spleen; severe congestion of large blood vessels and interstitial hemorrhages, vacuolation of glomeruli, coagulative necrosis of varying degrees in many of the renal tubular epithelial cells and infiltration of mononuclear cells in the interstitium of kidney and presence of irregular and elongated neurons in brain were observed in cyclophosphamide treated rats which were ameliorated by treatment with MEAM in a dose dependent manner. It is concluded from this study that CP administration @ 100mg/kg b wt, orally produced tissue changes that were ameliorated by MEAM (100 and 200 mg/kg b wt, orally for 28 days) in rats.
Ajuga macrosperma, Cyclophosphamide, Pathology, Rat
