Oocyte recovery and their quaility in relation to influence of corpus luteum

Experiment was conducted to assess the effect of presence of corpus luteum on ovarian follicular population, oocyte recovery and their quality in buffaloes. A total of 300 ovaries were collected, 165 were bearing corpus luteum (CL) and 135 without CL. A significantly (P<0.05) more number of oocytes were recovered (72.96%) from the ovaries without corpus luteum compared to ovaries bearing CL (67.42%). Usable oocytes (grade A and grade B) could also be recovered from ovaries without corpus luteum (531) as compared to ovaries bearing CL (363).Recovery of usable oocytes per ovary was 3.22±0.1 and 2.69±0.08 from the ovaries without CL and with CL, respectively. Present experiment concluded that presence and absence of corpus luteum (CL) on the ovaries has significant impact on follicular development as well as quality of oocyte recovered, thus this could be use to predetermine the recovery and qualities of oocytes in in vitro studies using such materials.