Acute oral toxicity study of anilofos in wistar Rats

The oral maximum tolerated dose (MTD) of anilofos in rat was determined in pilot dose range finding study following the standard method. Rats were observed for toxic signs and symptoms after oral administration of MTD of anilofos at single dose. The body weights of animals were recorded on alternate day for 14 days. Animals were sacrificed after 48 hrs and 14 day and changes in hematological parameters (Hb, TEC, TLC and DLC) and relative organ weight of various body organs (heart, liver, spleen, kidney, testis, epididymis and brain) were evaluated. The oral MTD of anilofos in rat was determined to be 750 mg/kg body weight. The sign and symptoms of acute toxicity shown by animals were dull, depress, respiratory dysponea, tremor and altered gait. Animals also showed ataxia, stiffness and fasciculation of muscles, head drop and protrusion of eye ball. Anilofos treatment resulted in decreased body weight gain as compared to the control group up to 14 days. The changes in hematological parameters were not significant between anilofos treated and control groups in both 48 hrs and 14 day study. The relative organ weights of liver and spleen is significantly differ at 48 hrs study and in 14 day significant difference was observed only in liver of treated group as compared to control group. MTD of anilofos in rat through oral route was determined for the first time. Study revealed a non-toxic delayed effect of anilofos on body weight, relative organs weight and hematological parameters in rat.